Simultaneous multiple target detection in real-time loop-mediated isothermal amplification
نویسندگان
چکیده
منابع مشابه
Simultaneous multiple target detection in real-time loop-mediated isothermal amplification.
Loop-mediated isothermal amplification (LAMP) is a rapid and reliable sequence-specific isothermal nucleic acid amplification technique. To date, all reported real-time detection methods for LAMP have been restricted to single targets, limiting the utility of this technique. Here, we adapted standard LAMP primers to contain a quencher-fluorophore duplex region that upon strand separation result...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2012
ISSN: 0736-6205,1940-9818
DOI: 10.2144/0000113902